TOP1MT knockout cell line (HeLa)

TOP1MT knockout cell line (HeLa) Order

Catalog#

YKO-H1259

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TOP1MT knockout cell line (HeLa)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：4

Species

Human

Gene Name

TOP1MT

Gene ID

116447

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 2 (based on transcript TOP1MT-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HeLa			STR Info (Cell bank) Cell Line:HELA
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	12	13.3		12	13.3
D7S820	8	12		8	12
D16S539	9	10		9	10
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	8	12		8	12
CSF1PO	9	10		9	10
D12S391	20	24	25.0
FGA	21	21
D2S1338	17	17
D21S11	27	28
D18S51	16	16
D8S1179	12	13
D3S1358	15	18
D6S1043	18	19
PENTAE	7	17
D19S433	13	14
PENTAD	8	15
D1S1656	12	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TOP1MT gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TOP1MT gene knockout TOP1MT knockout cell line (HeLa) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

TOP1MT knockout cell line (HeLa)

Catalog#

YKO-H1259

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Cervical Cancer Cell Line(Hela)
inquiry More details

Catalog#: YC-A012

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

TOP1MT Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: TOP1MT

Gene ID: 116447

Size: 1*10^6

Catalog#: YKO-XN47115

YKO-XN47115(HCT 116)
TOP1MT knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: TOP1MT

Gene ID: 116447

Size: 1*10^6

Catalog#: YKO-H1258

YKO-H1258(HEK293)
TOP1MT knockout cell line (A549)
inquiry More details

Organism: Human

Gene Name: TOP1MT

Gene ID: 116447

Size: 1*10^6

Catalog#: YKO-H1256

YKO-H1256(A549)

Related Popular Products

Wild-type Cell Lines

Human Cervical Cancer Cell Line(Hela)

Price (USD): -

Catalog#: YC-A012

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

TOP1MT Knockout cell line (HCT 116)

Organism: Human

Gene Name: TOP1MT

Gene ID: 116447

Size: 1*10^6

Catalog#: YKO-XN47115

inquiry More details
TOP1MT knockout cell line (HEK293)

Organism: Human

Gene Name: TOP1MT

Gene ID: 116447

Size: 1*10^6

Catalog#: YKO-H1258

inquiry More details
TOP1MT knockout cell line (A549)

Organism: Human

Gene Name: TOP1MT

Gene ID: 116447

Size: 1*10^6

Catalog#: YKO-H1256

inquiry More details

TOP1MT knockout cell line (HeLa)

Ubigene